抗菌肽将成对付超级病菌的新武器

The ever-increasing threat from "superbugs" -- strains of pathogenic bacteria that are impervious¹（不受影响的） to the antibiotics ² that subdued ³ their predecessor⁴（前辈） generations -- has forced the medical community to look for bactericidal weapons outside the realm of traditional drugs. One promising ⁵ candidate is the antimicrobial peptide (AMP), one of Mother Nature's lesser-known defenses against infections, that kills a pathogen by creating, then expanding, nanometer-sized pores in the cell membrane ⁶ until it bursts. However, before this phenomenon can be exploited as a medical therapy, researchers need a better understanding of how AMPs and membranes ⁷ interact at the molecular ⁸ level. Using a novel imaging technique, a research team led by the United Kingdom's National Physical Laboratory (NPL) is helping ⁹ acquire much-needed insight into the fundamental physical and chemical processes that occur when AMPs bind ¹⁰ with membranes and form pores in them. Team leader Paulina D. Rakowska will discuss the latest aspects of this work during the AVS 60th International Symposium ¹¹ & Exhibition, which will be held Oct. 27-Nov. 1, 2013, in Long Beach, Calif., ,Observing the formation of pores in live cell membranes by naturally occurring AMPs is difficult because researchers have no control over the steps in the complex process. In many cases, the membranes of the target cell leak, swell ¹² and rupture ¹³ before individual pores can expand enough to be examined. Rakowska and her colleagues have overcome this obstacle by combining nanoscale imaging via two different systems, computer simulation, a made-from-scratch (de novo) AMP, and lipid bilayers fixed ¹⁴ to a solid surface (known as a supported lipid bilayer or SLB)., ,With the ability to specifically test where and how the de novo peptide binds ¹⁵ to the SLB, the pore formation process is opened up to direct observation. Atomic force microscopy (AFM) provides topographical（地志的） (structural) imaging of the peptide-treated membrane while chemical analysis is done with high-resolution nanoscale secondary ion mass spectroscopy (NanoSIMS)., ,"Data from the AFM images suggests that membranes change as a result of peptide action and pore formation," Rakowska says. "NanoSIMS imaging performed on the same samples reveal the precise location of peptide molecules ¹⁶ within the membranes.", ,Rakowska says that these observations provide the first-ever physical and visual evidence of antimicrobial pore expansion from nano-to-micrometer scale to the point of complete membrane disintegration ¹⁷. "We can now postulate ¹⁸ the mechanism ¹⁹ by which this occurs," she explains. "We believe that the first AMPs binding ²⁰ with the membrane actively ²¹ 'recruit' others to do the same, resulting in the formation of numerous small pores. As these pores expand, they eventually lead to membrane disintegration（瓦解
，崩溃） and cell death.", ,The research team includes scientists from the NPL, the London Centre for Nanotechnology, University College London, the University of Oxford ²², the University of Edinburgh, Freie University Berlin and IBM. The team's latest publication, "Nanoscale imaging reveals laterally ²³ expanding antimicrobial pores in lipid bilayers," recently appeared in the Proceedings ²⁴ of the National Academy of Sciences USA.