蛋白质对维持β细胞功能有重要作用

Researchers at the Pediatric Diabetes ¹ Research Center (PDRC) at the University of California, San Diego School of Medicine have shown that the pancreatic（胰腺的） protein Nkx6.1 -- a beta-cell enriched transcription factor -- is essential to maintaining the functional ² state of beta cells. Type 2 diabetes is characterized by impaired ³ insulin secretion ⁴ by pancreatic beta cells in response to a rise in blood glucose⁵（血糖） levels. The study, published in the September 26 edition of Cell Reports, shows that loss of NKx6.1 in mice caused rapid onset ⁶ diabetes., ,UC San Diego scientists -- led by PDRC director Maike Sander, PhD, professor in the UCSD Departments of Pediatrics and Cellular ⁷ and Molecular ⁸ Medicine -- studied the molecular mechanisms ⁹ that underlie ¹⁰ loss of beta cell functional properties, such as regulated insulin secretion, during the progression of type 2 diabetes. They concluded that -- by impairing ¹¹ beta cell function -- reduced Nkx6.1 levels, as seen in type 2 diabetes, could contribute to its pathogenesis（发病机理）., ,Inactivating ¹³ the Nkx6.1 transcription factor in adult mice, then conducting a genome-wide analysis of Nkx6.1-regulated genes ¹² and functional assays ¹⁴, the scientists revealed the critical role of this protein in the control of insulin biosynthesis, insulin secretion and beta cell proliferation. Their findings demonstrate an intricate link between the beta cell's ability to import glucose, supporting an emerging concept that glucose metabolism ¹⁵ plays a critical role in beta cell proliferation., ,"We found the loss of Nkx6.1 activity had an immediate ¹⁶ and dramatic impact on the expression of genes that give beta cells their ability to synthesize and release insulin in a regulated fashion," said Sander. They discovered that genes involved in insulin biosynthesis, glucose import and glucose metabolism are direct transcriptional target genes of Nkx6.1. Its ablation also indirectly ¹⁷ impacted the expression of numerous genes important for the function and proliferation of beta cells., ,Over time, a subset of Nkx6.1-deficient beta cells acquired the molecular characteristics of somatostatin-producing delta ¹⁸ cells, suggesting a link between impaired beta cell function and loss of cell identity. However, such conversion ¹⁹ into delta and other types of non-beta cells was not observed when Nkx6.1 was inactivated ²⁰ at the embryonic ²¹ stage. Instead, a sequential loss of beta cell traits preceding the adoption ²² of alternative endocrine cell fates was observed after adult Nkx6.1 inactivation ²³, which closely mirrors the gradual loss of functional beta cell mass previously ²⁴ observed in models of type 2 diabetes., ,"Given that levels of Nkx6.1 are also reduced in human type 2 diabetic beta cells, our study lends support to the growing concept that loss of beta cell features could contribute to the onset of diabetes," Sander said.